ASTM D is a standard test anaerobic biodegradation test method for determining anaerobic biodegradation of plastic materials under high-solids. ASTM D Standard Test Method. D Standard Test Method for Determining Anaerobic Biodegradation of Plastic Materials Under. High-Solids. ASTM D Significance and Use. Biodegradation of a plastic within a high- solids anaerobic digestion unit is an important phenomenon because it will affect .

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This test method determines the degree and rate of biodegradation of plastic materials in an oxygen-free anaerobic qstm. Biodegradation of a plastic within a high-solids anaerobic digestion unit is an important phenomenon because it will affect the decomposition of other waste materials enclosed by the plastic and the resulting quality and appearance of the digestate after an anaerobic digestion process.

The procedure is intended to create a standard laboratory environment that will permit a rapid and reproducible determination of the anaerobic biodegradability under high-solids digestion conditions.

Placing the sample materials wstm an inoculum derived from anaerobic digesters operating on household waste.


This is often sludge from a water treatment plant mixed with a certain percentage of solid material from composting facilities. This inoculum contains methanogenic bacteria that are also found in landfills.

Placing the samples in incubation vessels after the inoculum is prepared. They are kept within strict temperature and moisture tolerances.

ASTM D5511 – Anaerobic Biodegradation

The vessels are airtight and are not exposed to UV radiation. Three vessels are used for each sample, and one awtm control and one positive control are prepared as well. Depleting the oxygen in the incubation vessels after an initial burst of aerobic activity. Then, the anaerobic bacteria begin to multiply and colonize in the vessel. As these bacteria begin to utilize the carbon in the test samples, they produce carbonaceous gasses such as Asfm and CH4.

These gasses are awtm daily and the results are carefully recorded. The test is allowed to run for as long as desired. If the positive control which contains cellulose continues to exhibit biodegradation then the test is considered valid and the inoculum considered alive.


When the test is run to satisfaction, final carbon and methane gas readings are recorded and the incubation vessels are emptied and the samples are cleaned and weighed. The molar weight of the total gas produced by the test samples is calculated and compared to the carbon weight of the original samples and the percent biodegradation of the samples can then be determined. This test d511 is designed to be applicable to d55511 plastic materials that are not inhibitory to the microorganisms present in anaerobic digesters operating on household waste.

BioPro Global – ASTM D

Reports shall clearly state the percentage of net gaseous carbon generation for both the test and reference samples d5151 the completion of the test. Furthermore, results shall not be extrapolated past the actual duration of the test. The values given in SI units are to be regarded as the standard. The testing process includes:

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