GARZON, Luz Nayibe; BLAIR, Matthew W. LIGARRETO, Gustavo A.. Use of molecular marker assisted selection for resistance to anthracnose in common beans. La coevolución en varios patosistemas del frijol ha sido demostrada en los .. Poblaciones de C. lindemuthianum agente causal de la antracnosis del frijol. Recomendaciones y acuerdos del primer taller de antracnosis en América Latina . In: La Antracnosis del Frijol Común, Phaseolus vulgaris, en América Latina.

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The pathogenic variability of the fungus Colletotrichum lindemuthianum represents an obstacle for the creation of resistant common bean Phaseolus vulgaris L. Gene pyramiding is an alternative strategy for the development of varieties with durable resistance.

RAPD markers have been proposed as a means to facilitate pyramiding of resistance genes without the need for multiple inoculations of the pathogens. The two progenitors, populations F 1 and F 2F 2: The results indicate that a single dominant gene, Co-6controls common bean resistance to this race, giving a segregation ratio between resistant and susceptible plants of 3: The segregation ratio of F 2: The common bean Phaseolus vulgaris L.

Fungal diseases drastically affect common bean yield, and anthracnose, caused by the fungus Colletotrichum lindemuthianum Sacc. Although breeding the common bean for resistance to anthracnose by traditional methods has created several resistant cultivars Singh et al. Gene pyramiding is an alternative strategy for the development of varieties with durable resistance, but traditional breeding procedures are inefficient for such purpose due to the difficulties brought about by the need for multiple inoculations Michelmore, Pyramiding of resistance genes assisted by molecular markers has been proposed as an alternative solution for this type of problem Haley et al.

A number of common bean lines have been evaluated for resistance to many pathotypes of anthracnose at the Centro Internacional de Agricultura Tropical CIAT, Cali, Colombiaand among them, cultivar ABselected as one of the 12 international differential cultivars for anthracnose, is one of the most resistant Pastor-Corrales, Cultivar AB showed resistance to 25 pathotypes of C. This technique is being used in our common bean breeding program to facilitate the development of isolines containing disease resistance genes of interest.


Race 89 of C. The original inoculum was kindly provided by Dr. To increase the amount of spores the isolates were cultivated for approximately 10 days in sterile medium containing common bean green pods.

To confirm the identity of the isolates, they were inoculated on the 12 bean anthracnose differentials according to the method of Pastor-Corrales The populations were maintained in the greenhouse. The following number of seeds were sowed in the greenhouse in a completely randomized design: Fourteen days after sowing the first expanded trifoliate leaf from each of the plants was inoculated on the lower and upper leaf surfaces with spore suspensions 1.

Resistant R phenotype was assigned to plants with no or limited symptoms grades 1 to 3whereas plants graded 4 or greater were considered to be susceptible S. Before inoculation, primary leaves from all plants were collected and kept at o C for DNA extraction.

The phenotypic class frequencies obtained were tested for goodness-of-fit to theorical ratios with chi-square tests. One hundred and eighty-eight F 2: Inoculation conditions and symptom evaluation were performed as before except that in this case spores were sprayed onto the plants with the aid of a De Vilbiss apparatus.

DNA extraction was according to Doyle and Doyle Each reaction 25 m l contained: Each amplification cycle consisted of one denaturation step at 94 o C for 15 s, one annealing step at 35 o C for 30 s and one extension step at 72 o C for 1 min. After 40 cycles an extra extension step was performed for 7 min at 72 o C. Amplification products were analyzed on 1. Chi-square analysis was used to test the phenotypic segregation of the F 1F 2F 2: To determine the genetic distance between the RAPD marker and the resistance gene, F 2 plants were used.

The segregation ratio resistant: This gene was identified in cultivar Catrachita, a breeding line derived from AB and named Co-6 Young and Kelly, b. Similar results were reported in previous work using the same resistance source Vidigal, ; Alzate-Marin et al. Co-6 has proven to be effective against a large number of Latin American isolates of C.

Using this marker it was possible to confirm that a single gene or a complex locus of tightly linked resistance genes present in cultivar AB confers resistance to C. The OPZ04 marker could be used in a marker-assisted breeding program to pyramid different resistance genes using cultivar AB as a source for anthracnose resistance.


The presence of this marker in cultivar Catrachita Figure 3 antrancosis supports the idea that the gene studied in this work is the same Co-6 as that reported by Young and Kelly b. To determine if the other 11 differential cultivars carry the molecular marker OPAZ20 linked to the Co-6 gene in cultivar ABthe DNA of these cultivars was extracted and amplified with the appropriate primers.

Anthracnose on bean.

The allelism studies conducted so far do not indicate the presence of alternative alleles for Co-6 in other differential cultivars for anthracnose Young and Kelly, b. Menarim a fellowship from CNPq. A single gene or complex locus of linked resistance genes confer resistance to four pathotypes of Colletotrichum lindemuthianum in common bean. Backcross assisted by RAPD markers for the introgression of Co-4 and Co-6 anthracnose resistant genes in common bean cultivars. Isolation of plant DNA from fresh tissue.

Identification de RAPD markers linked to a major rust resistance gene block in common bean.

Selection for monogenic resistance traits with coupling and repulsion-phase RAPD markers. Two new molecular markers linked to bc An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Molecular approaches to manipulation of disease resistance genes.

La antracnosis del fríjol y su control

Anrtacnosis of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Enfermedades del frijol causadas por hongos. Antracnose do feijoeiro no Estado de Minas Gerais-Brasil. Breeding common bean for resistance to anthracnose. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

RAPD markers flanking the Are gene anteacnosis anthracnose resistance in common bean. Characterization of the genetic resistance to Colletotrichum lindemuthianum in common bean differential cultivars. RAPD markers linked to three major anthracnose resistance genes in common bean. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License.

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